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Describe the use of promoters in gene technology

Promoters switch genes on, if a gene is inserted into a bacterium it needs a promoter added to coordinate with the gene for the chanracteristic to be expressed.

For human insulin production in bacteria:

1) Gene intersted next to the B-galactosidase (lactase) gene so that they share a promoter.

2) Bacteria are grown in a medium containing lactose.

3) Lactase gene is switched on by promoter which switches on the insulin gene at the same time.

4) B-galactosidase and Human insulin are synthesised by the Bacteria, excreted and harvested for medical use and research. 

Roslyn I. GCSE English tutor, GCSE Biology tutor, A Level Extended Pr...

3 years ago

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