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The preparation occurs to the DNA whilst in the nucleus of the cell.
As DNA is a double helix, the two strands of DNA need to be split apart. Therefore the hydrogen bonds need to be broken. The DNA helicase is the enzyme needed for these bonds to break.
Then the two strands are separated for the replication process to continue.
Primary succession starts from bare rock, so no soil is present at the start. Over time the pioneer organisms (such as moss and lichen) break the rock and as the organisms die and decompose, the soil builds up.
Secondary succession occurs when in an environment which had a little soil already. It happens when the previous community is lost, such as due to a forest firesee more