How could ionic interactions be used in DNA-RNA hybridisation?

The DNA double helix consists of many hydrogen bonds between the base pairs, but in addition there are negatively charged phosphate molecules as a key component of the sugar-phosphate backbone of DNA strands. In order to induce the hybridisation between a single stranded DNA molecule and a complementary single RNA strand, the double helix must be separated. This is done by introducing ions such as Na+ and Mg2+; as these are positively charged ions, they will interact and partially counteract the phosphates negative charge. Having these ions present in a water solution, will also allow the hydrogen bonds between the bases to be temporarily relocated to having formed hydrogen bonds with the water solution.

MA
Answered by Mohammed A. Biology tutor

1330 Views

See similar Biology University tutors

Related Biology University answers

All answers ▸

Describe how a cell produces a mature protein from DNA, including the main enzymes involved and where each stage takes place.


How is a resting membrane potential of a neuron maintained?


Explain the pathway of MPS following resistance exercise?


What is ocean acidification?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2025 by IXL Learning