How would you test for the presence of a non-reducing sugar?

We can check for the presence of non-reducing sugars the Benedick's Test. If a reducing sugar is present in a solution, adding Benedick's reagent and heating will form an insoluble red precipitate. Non-reducing sugars do not change the colour of the solution, which is blue, and so we have to break the sugar down to monosaccharides by hydrolysis to prove they're non-reducing. So, when the Benedick's test gives a negative result, add dilute hydrochloric acid and put this in a water bath; this will hydrolise the bonds between the disaccharides. Hydrogen carbonate is then added to neutralise the solution as Benedick's reagent doesn't work under acidic conditions. Re-do the Benedick's test- if a non-reducing sugar was present, the solution will now produce a red precipitate, if no no reducing or non-reducing sugars were present, the solution will remain blue.

JO
Answered by Jess O. Biology tutor

109424 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

What is the difference between Tumour supressor genes and Oncogenes?


Guillain-Barré syndrome and multiple sclerosis both cause muscle weakness and loss of muscle function. Suggest and describe how the function of neuromuscular junctions will be affected by Guillain-Barré syndrome and multiple sclerosis.


What is transpiration?


How does information from a gene make a protein?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2025

Terms & Conditions|Privacy Policy
Cookie Preferences