How would you test for the presence of a non-reducing sugar?

We can check for the presence of non-reducing sugars the Benedick's Test. If a reducing sugar is present in a solution, adding Benedick's reagent and heating will form an insoluble red precipitate. Non-reducing sugars do not change the colour of the solution, which is blue, and so we have to break the sugar down to monosaccharides by hydrolysis to prove they're non-reducing. So, when the Benedick's test gives a negative result, add dilute hydrochloric acid and put this in a water bath; this will hydrolise the bonds between the disaccharides. Hydrogen carbonate is then added to neutralise the solution as Benedick's reagent doesn't work under acidic conditions. Re-do the Benedick's test- if a non-reducing sugar was present, the solution will now produce a red precipitate, if no no reducing or non-reducing sugars were present, the solution will remain blue.

JO
Answered by Jess O. Biology tutor

117279 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

During sampling, discuss the principles and strategies that should be employed in the collection of representative samples.


Describe and explain some of the direct and indirect effects of carbon dioxide on organisms?


what is the difference between homoeostasis and haemostasis


Can you explain the four levels of protein folding?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2026 by IXL Learning