Describe the process of PCR, including what is needed.

Chemical components needed: a DNA template, DNA polymerase enzyme, primers, nucleotides and reaction buffer.

Steps in PCR:

  1. Denaturation 96 degrees, to seperate DNA strands

  2. Annealing 55-65 degrees, cool so that primers can bind to their complementary sequences

  3. Extension 72 degrees, raise temperature so that polymerase can work to extend primers making new DNA

KS
Answered by Kavi S. Biology tutor

2667 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

What is the process of the heart pumping?


Aspartic Acid and Proline are both part of the 20 basic amino acids. Describe their similarities and differences in terms of their properties.


How does increasing temperature affect the rate of a reaction in the presence of an enzyme.


What is the difference between hydrophillic and hydrophobic molecules?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2025

Terms & Conditions|Privacy Policy
Cookie Preferences