What sort of inhibitor in an enzyme catalysed reaction is NOT affected by substrate concentration?

Non competitive inhibitors bind to a site other than the active site, called the allosteric site. As a result of this, the 3D tertiary structure of the enzyme is damaged and the enzyme is no longer capable of catalysing a reaction (remember: the tertiary structure damage means that the active site is also damaged and therefore, substrate-enzyme complexes can no longer be formed). Since these non competitive inhibitors do not compete with substrate molectules, non competitive inhibitors are not affected by substrate concentration (remember: even if you increase substrate concentration, the active sites on enzymes have been damaged and therefore, substrate can not attach to form complexes, the enzymes itself are damaged). 

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Answered by Aishwarya S. Biology tutor

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