Explain how a change in a sequence of DNA bases could result in a non-functional enzyme

A mutation in the DNA sequence may lead to a different amino acid sequence may lead to a different amino acid being produced in the primary protein structure of the enzyme during translation. This may cause errors in protein folding - causing the protein to form different three-dimensional tertiary structure. If this occurs in the active site of the enzyme, successful enzyme-substrate complexes may no longer be able to form