How did Meselson and Stahl provide evidence for semi-conservative replication?

One sample of bacteria was grown in a nutrient broth containing light nitrogen (N14) and one was grown in a broth with heavy nitrogen (N15). The bacteria took up the nitrogen to make new nucleotides so it was incorporated into their DNA. A sample of each was taken and centrifuged, and the DNA grown in the heavy nitrogen settled lower in the tube than the light nitrogen did. The DNA grown in the heavy nitrogen broth was taken and replicated again but this time in the light nitrogen. When centrifuged, it settled at a point in between where the heavy and light nitrogen did, showing that it kept a strand of the old DNA and created a new strand with the light nitrogen. Therefore, the DNA replicated semi-conservatively.

CS
Answered by Chloe S. Biology tutor

22242 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

Tom is an expactant father who is worried that he will pass on his Haemophilia A, an X linked recessive disease, to his offspring. The mother is homozygous dominant. Explain why his 1st generation offspring will not be affected by the condition.


What is mitosis?


Mitosis is the process of cell division, it occurs in humans and in plants. Compare and contrast the process of mitosis in human cells and in plant cells (5 marks)


Give two features of DNA AND explain how each one is important in DNA replication.


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2026 by IXL Learning