Describe the process of DNA replication

DNA replication is semi-conservative, meaning existing strands are used as templates for new strands. The DNA is separated (unwinds) by the enzyme helicase. This enzyme breaks the H bonds between nucleotides. Topoisomerase breaks the DNA ahead of the opening of the replication fork before it gets to tight (to reduce tension). An enzyme called primase starts a short RNA chain from scratch using RNA nucleotides. Now DNA polymerase can build the new DNA strand using complementary base pairing (Adenine - Guanine, Cytosine - Uracil) in the 5' to 3' direction.
The second strand is built up by joining segments called Okazaki fragments using the enzyme DNA ligase. This occurs in the 3' to 5' direction and makes phosphodiester bonds.
This results in the formation of 2 identical DNA complexes.

KO

Related Biology A Level answers

All answers ▸

What is the difference between mitosis and meiosis?


How does water play a vital role in sustaining life?


A plant community on an extinct volcano displays the process of succession. Explain why there would be very few species at the end of the eruptive life cycle of the volcano and why rate of new species introduction would rapidly increase afterwards.


Could you name and describe the different stages of the mitotic cell cycle?