Glycolysis takes place in the cytoplasm and is anaerobic (requires no oxygen). 2ATP molecules are needed for the phosphorylation of glucose, forming hexose phosphate. Hexose phosphate is unstable and forms two molecules of triose phosphate (TP). Each TP molecule loses two hydrogen atoms by dehydrogenation, catalysed by the enzyme dehydrogenase. The hydrogen atoms released are picked up by the nucleotide NAD (X on the diagram below) forming reduced NAD. Two molecules of ATP are also produced by substrate level phosphorylation (A) - pyruvate (3C) is formed. The products formed by glycolysis are pyruvate, reduced NAD and two molecules of ATP (net).