Describe the Benedict's test for reducing sugars and how this can be modified to test for non-reducing sugars

Take 1-2cm3 of the sample in a test tubeAdd a few drops of Benedict's reagent to the test tube Heat the test tube in a water bath Leave for 5 minutes Observe the colour of the precipitate formed
no precipitate formed = negative for reducing sugarsprecipitate formed = positive for reducing sugars(green -> yellow -> orange -> brick red) increasing concentration of reducing sugars present
modify for non reducing sugarstake a new sample in a clean test tube add dilute HClwarm (at a lower temperature) in a water bath remove from the water bath and add NaHCO3add Benedict's solution repeat steps 3-5 with this sample





JP
Answered by Jennifer P. Biology tutor

7903 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

What are the different protein structures?


Contrast facilitated diffusion and active transport


Explain how DNA is replicated and why this is important in biology.


Suggest how a wood mouse maintains a constant body temperature when in a cold environment?


We're here to help

contact us iconContact usWhatsapp logoMessage us on Whatsapptelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2025

Terms & Conditions|Privacy Policy
Cookie Preferences