Describe the Benedict's test for reducing sugars and how this can be modified to test for non-reducing sugars

Take 1-2cm3 of the sample in a test tubeAdd a few drops of Benedict's reagent to the test tube Heat the test tube in a water bath Leave for 5 minutes Observe the colour of the precipitate formed
no precipitate formed = negative for reducing sugarsprecipitate formed = positive for reducing sugars(green -> yellow -> orange -> brick red) increasing concentration of reducing sugars present
modify for non reducing sugarstake a new sample in a clean test tube add dilute HClwarm (at a lower temperature) in a water bath remove from the water bath and add NaHCO3add Benedict's solution repeat steps 3-5 with this sample





Answered by Jennifer P. Biology tutor

2684 Views

See similar Biology A Level tutors
Illustration of a video tutorial

Need help with Biology?

One to one online tuition can be a great way to brush up on your Biology knowledge.

Have a Free Meeting with one of our hand picked tutors from the UK’s top universities

Find a tutor

Related Biology A Level answers

All answers ▸

Explain how the alveoli create a surface for efficient gaseous exchange


What's the difference between a promoter, a primer and a probe?


Blue-spotted tree monitors (Varanus macraei) are found on the Indonesian island of Batanta. Describe how you could use the mark-release-recapture method to estimate the number of Varanus macraei on the island. (4 marks)


Draw a graph on the effect of an increase in temperature on the rate of an enzyme controlled reaction. Explain the shape of the curve and suggest why the temperature of a mammal is maintained just below the optimum temperature.


We're here to help

contact us iconContact usWhatsapp logoMessage us on Whatsapptelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2022

Terms & Conditions|Privacy Policy