What are the methods of immobilising enzymes?

  • Google+ icon
  • LinkedIn icon
  • 1377 views

There are four methods of enzyme immobilisation: adsorption, covalent bonding, entrapment and membrane separation.

Adsorption involves mixing the enyzme with an immobilsing support so the enzyme molecules will bind to it because of hydrophobic interactions and ionic links. The support is called an adsorbing agent and could be porous carbon, glass beads or clays.

Covalent bonds between enzyme molecules and an insoluble material, such as clay, are formed using cross-linking agents (e.g. sepharose).

Entrapping the enzyme molecules in a network or gel beads or cellulose fibres allows the enzyme to be immobilised in their natural state (not attached to another molecule). 

Membrane separation physicially separates the enzyme and substrate solutions with a partially permeable membrane. Both the substrate and product molecules are small enough to pass through the membrane.

Alice H. GCSE Biology tutor, GCSE Chemistry tutor, GCSE Maths tutor, ...

About the author

is an online A Level Biology tutor with MyTutor studying at Bristol University

Still stuck? Get one-to-one help from a personally interviewed subject specialist.

95% of our customers rate us

Browse tutors

We use cookies to improve your site experience. By continuing to use this website, we'll assume that you're OK with this. Dismiss

mtw:mercury1:status:ok