Explain how restriction endonucleases are used to form recombinant DNA plasmids.

Endonucleases cut DNA at palindromic sites producing 'sticky ends'. The endonuclease cuts the plasmid DNA and the DNA to be inserted at the same palindromic site. The sticky ends of the plasmid DNA and the insert DNA are complimentary and form hydrogen bonds. DNA ligase sticks the DNA together to form a recombinant DNA plasmid.

TD
Answered by Tutor392986 D. Biology tutor

2776 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

Explain what causes the Bohr shift to occur, and give one situation in which this is useful.


How many chromatids does a cell contain during Prophase?


How does the water potential in the medulla of the kidney remain lower than the water potential in the loop of Henle?


What is the difference between transcription and translation?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2025

Terms & Conditions|Privacy Policy
Cookie Preferences