Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA.

PCR is a way of producing large quantities of a specific target sequence of DNA.

It is useful when only a small amount of DNA is available for testing  (e.g. crime scene samples of blood, semen, tissue, hair, etc.).

 

PCR occurs in a thermal cycler and involves a repeat procedure of 3 steps:

1.  Denaturation:  DNA sample is heated to separate it into two strands

2.  Annealing:  DNA primers attach to opposite ends of the target sequence

3.  Elongation:  A heat-tolerant DNA polymerase (Taq) copies the strands 

 

- One cycle of PCR yields two identical copies of the DNA sequence

Answered by Zana Katarina L. Biology tutor

7616 Views

See similar Biology IB tutors
Illustration of a video tutorial

Need help with Biology?

One to one online tuition can be a great way to brush up on your Biology knowledge.

Have a Free Meeting with one of our hand picked tutors from the UK’s top universities

Find a tutor

Related Biology IB answers

All answers ▸

What are the differences between eukaryotic and prokaryotic cells?


Please explain patterns of sex linked genetic inhertitance and mention one or more examples of sex linked diseases


How can we reduce our impact on nature?


Explain Darwin’s theory of evolution by natural selection.


We're here to help

contact us iconContact usWhatsapp logoMessage us on Whatsapptelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

© MyTutorWeb Ltd 2013–2022

Terms & Conditions|Privacy Policy