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First thing: don't panic! I know it is scary, when you are in front of an long unseen translation and you don't seem to recognise any word. What you need to do is take a deep breath and start unravell...

Promoters switch genes on, if a gene is inserted into a bacterium it needs a promoter added to coordinate with the gene for the chanracteristic to be expressed.
For human insulin production in bacter...

Personally, I find that the easiest way to know when to use the subjunctive is by using the acronym WEDDING.

W- wish/will

e.g. querer que, desear que, preferir que. Mi amigo quiere...