How does DNA replication work?

DNA replication is called semi-conservative, because each original strand is used to create a new strand. In order to do this, first we need to separate the two strands: they are unwound and unzipped by the enzymes DNA gyrase and DNA helicase, to leave two separate strands with the nitrogenous bases exposed. Next, free activated nucleotides are "matched" to the complementary exposed bases on each strand (A to T, C to G). The enzyme responsible for this is called DNA polymerase. Hydrogen bonds form between the complementary bases, and DNA polymerase ensures a sugar phosphate backbone forms on the new strand. However, DNA polymerase works in only one direction, and we know that DNA strands run antiparallel (one 3' to 5' and one 5' to 3'). On the strand where DNA polymerase is not able to smoothly move down the strand (because it keeps hitting un-separated DNA), new DNA forms in small chunks called Okazaki fragments, which are joined by a DNA ligase enzyme.

ET
Answered by Ellie T. Biology tutor

3595 Views

See similar Biology A Level tutors

Related Biology A Level answers

All answers ▸

What is the role of ATP in muscle contraction?


Is all of a persons DNA used to create the body?


Explain how a plant is able to make glucose from carbon dioxide.


how is urine produced in the kidney?


We're here to help

contact us iconContact ustelephone icon+44 (0) 203 773 6020
Facebook logoInstagram logoLinkedIn logo

MyTutor is part of the IXL family of brands:

© 2025 by IXL Learning